Analysis of Gene Expression with SuperSAGE:
SuperSAGE defines what gene is how often transcribed by sequencing and counting millions of unique short sequences ("tags") from each transcript. SuperSAGE is a substantially improved version of the SAGE and LongSAGE method. By using a highly precise 26 bp tag instead of the 14-18bp long tag,
in combination with new sequencing technologies, SuperSAGE overcomes the two major drawbacks of the predecessor techniques: low accuracy and high costs.
What Gene is expressed and how often?
By high-throughput sequencing and counting of millions of unique 26bp tags of transcript-cDNA, SuperSAGE analyzes any eukaryotic transciptome at an ultra-deep scale at a very competitive price.
Accuracy of the 26 bp vs. 18 bp-tag:
Destinct results only with 26 bp SuperSAGE tags
