RNA-Seq

RNA-Seq is a Next Generation Sequencing (NGS) approach for the qualitative and quantitative analysis of entire transcriptomes. Fragmented cDNA is sequenced by NGS. The resulting sequences can either be assembled for non-model organisms, or mapped to known genomes. Quantification of the RNAs or contigs is achieved by counting the corresponding fragments. As full-length transcripts can be analyzed, the technique allows identifying splice-variants and SNPs.
Bioinformatics is calculation- and therefore labor-intensive, and datasets are immense. To analyse rare transcripts, very deep sequencing is required (~100 mio reads of 100 bps).

GenXPro's RNA-Seq service includes:

• preparation of the library using patented technique for bias free quantification
  
• Sequencing with platform of choice
• Assembly using MIRA or Velvet
• Bias-free quantification of transcripts/tags using patented method
  by mapping to contigs or database entries

Required Material: 1-10 microgram of total RNA

Turnaround time: 1-3 month.