RT PCR Details

 

The GenXPro RT PCR setup can be used for the validation of SuperTag and SuperTag array data, expression analysis of any gene, quantification of microRNAs, and detection of SNP polymorphisms in genes.

 

 

  • Confirmation of representative SuperTag and SuperTag array data, quantitative evaluation of up- and down-regulated transcripts. We deliver genome-wide SuperTag gene expression profiles together with qRT-PCR-confirmed sets of transcripts.

 

  • Full quantitative PCR (qPCR) service for quantitative expression analysis of any gene of your interest. Just send your RNA, and we analyse it, and provide you with a compilation and interpretation of the data ready for publication.

 

  • Fast and quantitative monitoring of gene knock-downs by small interfering RNAs (siRNAs).

 

  • Quantitative analysis of mature and active microRNAs (miRNAs), using target-specific primers with a stem-loop conformation, that differentiate between members of a miRNA family.

 

  • Quantification of allelic expression of specific genes and detection of gene copy changes in agriculture and medicine.

 

  • Detection of single nucleotide polymorphisms (SNPs) in genes by TaqManTM assays for plant, animal and human genotyping and analysis of genetic diseases.

 

 

Price details upon request

 

 

 GenXPro offers the TaqMan™ assay and the less stringent SYBR Green intercalator assay.



StepOne Real Time PCR System

 

 

RT-PCR (reverse transcription -polymerase chain reaction) currently is the most sensitive technique for mRNA detection and quantitation. The two other competing techniques, Northern blot and RNase protection analyses, require much higher mRNA input levels than RT-PCR. In fact, RT-PCR is as sensitive as to quantitate RNA from a single cell.

 

GenXPro GmbH Altenhöferallee 3, D-60438 Frankfurt am Main, tel. +49 (0)69-95739710, fax. +49 (0)69-95739706, e-mail: info@genxpro.de

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