Methlyation of CpG islands and promotors...

...can be elegantly and efficiently analysed by employing methylation sensitive or methylation dependent restriction enzymes, who will digest the DNA according to its methylation state.

GenXPro produces methylation-specific fragment libraries which are sequenced in their millions to analyse genome wide methylation patterns of any genome. Our PCR-bias free data can accurately quantify differences in the methlyation state. The resolution of the analysis can be adapted by the choice of the restriction enzyme, e.g.,  a four base cutter will digest in theory every 256th base pairs, while a six base cuter only every 4096 base pairs.

 The scheme  below shows the procedure, the M-marked regions are methylated and will not be digested by the methylation sensitive restriction enzyme.