Digital Gene Expression SuperTAG Service:
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New transcripts, rare transcripts, sense transcripts, anti-sense transcripts
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Exact transcript quantification
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Easy annotation of tags to genes in databases
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No false positives
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No limitation to sequenced genomes
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Tags directly applicable as primers for full-length cDNA amplification
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Interchangeable data
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GO enrichment analysis: concerned molecular functions, cellular components and biological processes are identifed using our STDGE2GO software.
You send:
At least 10 microgram total RNA or the equivalent amount of cells or tissue
You receive: An excel-table containing the expression profile from three to ten million 26 bp SuperTAGs. The digital gene expression data show how many times the individual tag was present, the annotation to known sequences in a publicly available database, the ratio and p-value stating the level of and probability for the differential expression of each tag. Additionally, a Gene Ontology enrichment analysis identifes the concerned molecular functions, cellular components and biological processes using our STDGE2GO software (please click for a test-login).
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Limited offer:
GenXPro is looking for cooperations and offers a 30% cutoff for selected reference projects in diverse areas of life sciences.
For more information please contact us.
Normalization of cDNA, Sequencing and Assembly
GenXPro offers a full service of cDNA preparation, normalization, sequencing and assembly of cDNAs. We have developed optimized methods for the preparation of normalized cDNA libraries, avoiding 3’ and 5’ end biases and eliminating as much frequent transcripts as possible, without losing the information of rare transcripts.
You send: 10 -50 µg total RNA
You receive: 2 x 8-12 million 75 bp Solexa reads, Bioinformatics (assembly) and statistics, cluster analysis, SNP detection, statistics.
Massive analysis of normalized cDNA ends (MANCE):
MANCE is a highly efficient approach for the analysis of polymorphisms (mostly SNPs) in 3' transcript ends of any organsim. In contrast to polymorphisms somewherei in the genome, any polymorphism in a transcript can be related and mapped with much higher chance to a phenotype or QTL. The 3’ UTRs of transcripts contain a higher number of SNPs than the coding region. The MANCE approach threfore targets only this region. By normalization, the very frequent transcripts are reduced, to maximize the SNP detection.
Our service comprises the entire process, starting with total RNA. The data output will contain all detected cDNA end, as well as possible SNPs and polymorphisms of at least two datasets.
You send: 5 -50 µg total RNA
You receive : at least 125 000 thousand 454 reads of 100-400 bp, cluster analysis, SNP detection, statistics
Or: 5-15 million 38 bp Solexa reads, cluster analysis, SNP detection, statistics
Marker and Chip Technology:
GenXPro develops and applies diagnostic and prognostic genomic, genic and expression markers for all eukaryotic organisms.
